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  • Capillary refill time is used to assess peripheral perfusion. In a healthy, well-perfused animal, capillary refill time is typically what?
  • How do cells absorb nutrients from the blood?
  • What is a vaginal plug?
  • What temperature range is commonly used in many facilities as stated in the material?
  • Which reason best explains why rodents and rabbits are not fasted prior to surgery?
  • Which organization is responsible for monitoring compliance with Public Health Service Policy?
  • What structure lies between the vertebrae?
  • What procedure is recommended for maintaining an emergency cart?
  • Where are catheters placed?
  • If a researcher uses fruit flies instead of mice for a study, which 3Rs principle is being applied?
  • Which heart chamber has the thickest wall?
  • Which organization uses the Guide for the Care and Use of Laboratory Animals (the Guide) as one of its standards for the accreditation of laboratory animal programs?
  • What type of breeding is being used when the males remains with the female or females continuously?
  • Through what process does oxygen and carbon-dioxide exchange occur between the blood and the air?
  • Which lymph nodes are found in the armpit area?
  • Which of the following categories of disease lasts for the longest time?
  • Which statement about arterial catheterization is true?
  • A subcutaneous injection is administered in which tissue region?
  • What tissue is the trachea made from?
  • Which term describes an organism composed of cells from two different zygotes?
  • Total hardness is a measure of which ions?
  • What occurs after birth in rat, mice, and guinea pig females?
  • Between which structures is the intervertebral disc located, as described in the material?
  • Which of the following is a form of structural enrichment?
  • Which shape is used to describe the rodent kidney in the source material?
  • What is the usual treatment for an abscess in a laboratory animal?
  • What term refers to the cause or origin of a disease?
  • In budgeting for animal care, which cost category stays constant across the budget period?
  • Which component is primarily involved in the fermentation of roughage in ruminants?
  • What is the period of time called that a chemical is left on a surface before it is rinsed?
  • How much does a 30 lb dog weigh in kg? (Conversion factor: 2.2 lb/kg)
  • When is the pathogenic microorganism Mycoplasma pulmonis, more likely to cause disease?
  • Which statement about recordkeeping is true?
  • Which statement is true regarding emergency care?
  • After topical parasite treatment, poisoning signs should be addressed with which initial step?
  • Which statement describes a coisogenic animal?
  • Which statement about the endocrine system is true?
  • Which term describes an organism composed of cells from two different zygotes?
  • What is a transgenic mouse?
  • For how long must minutes of IACUC meetings and semi-annual inspection reports be kept?
  • Which term describes the administration of a liquid directly into the stomach via a tube placed in the esophagus?
  • Which of the following regulates disinfectants and sterilizers used on hard surfaces?
  • Which device is used for long-term, continuous infusion in unrestrained animals?
  • What role do AVMA guidelines play in laboratory animal euthanasia?
  • What type of water is essential for aquatic species?
  • To draw into a syringe 100 mg of a preparation at a concentration of 25 mg/mL, what volume is needed?
  • What does the severity of a disease depend on?
  • Which blood cell type carries oxygen in the bloodstream due to hemoglobin?
  • Suture materials are sized by which property?
  • Which statement is NOT true of a decentralized management program?
  • Mastitis primarily affects which anatomical structure?
  • What do thrombocytes do?
  • Which term describes a disease that is similar to an endemic in humans?
  • What does preclinical mean in drug development?
  • What is included in a surgical field?
  • A local disease is best described as a disease that:
  • Which term describes an organism whose body contains a mixture of cells, some with a foreign gene and some without?
  • In drug development, which phase directly follows in vitro testing?
  • How many heart chambers do mammals have?
  • Why is it important not to rush when scrubbing down fingers, hands, and arms?
  • How often does the Guide specify the IACUC should review the entire animal care and use program including an inspection?
  • Which statement best describes the purpose of a booster injection?
  • Which process involves fertilizing eggs outside the body before transferring them to a recipient female?
  • What is relative humidity?
  • To comply with the Animal Welfare Act, a facility must follow which regulatory framework?
  • What is the true stomach called in the ruminant?
  • From the main airways, the branching tubes become progressively smaller. What are these smallest conducting airways called?
  • Which practice helps ensure a rodent's weaning cage setup supports pups’ access to resources?
  • Under the Animal Welfare Act, which element is explicitly required for dogs?
  • What is the individual when both genes at the same locus on a chromosome pair are different?
  • What is occurring when lymph nodes become swollen and painful?
  • What is the name of a cell produced at fertilization by the union of sperm and egg?
  • What is the cytoplasm?
  • The route of infection is best defined as:
  • What is cystocentesis?
  • Why is it important to provide an external heat source during and after surgery?
  • Cost analysis is primarily used to determine what?
  • What is the outer layer of skin?
  • Which type of chemical kills all microbial life and spores?
  • What term refers to assigning less complicated tasks to others in order to allow supervisors more time to get other work completed?
  • During intravenous procedures, how should proximal pressure be handled?
  • Which animal is required by the Animal Welfare Act to have psychological enrichment?
  • Which dental instrument is used to remove calculus from teeth?
  • Which concept explains why two genes located close to each other on the same chromosome are often inherited together?
  • Which record is needed to calculate per diems?
  • What is checked when monitoring perfusion during anesthesia in a mouse?
  • How long does it take to establish a healthy biologic filter with the appropriate bacteria?
  • Which term describes a disease that persists over a long period?
  • What should you do when setting up a rodent weaning cage?
  • Which statement about HEPA filtration is consistent with the material?
  • According to the Guide, which procedure is defined as one that "penetrates and exposes a body cavity, produces substantial impairment of physical or physiologic functions, or involves extensive tissue dissection or transection"?
  • Where is roughage broken down in a goat?
  • What are the wave-like contractions of the esophagus that help move food down the esophagus called?
  • Regarding glass bead sterilization, which statement is true?
  • Regarding shock, which statement best describes its effect on circulation?
  • Which statement correctly defines a major surgical procedure?
  • What is the recommended treatment schedule when administering an insecticide?
  • What are different versions of the same genes called?
  • The IACUC reviews animal use protocols to ensure compliance with which levels of law and policy?
  • Before you start on a task, it is important to:
  • A monogamous mating describes which pairing?
  • How is the molecular weight determined for a molecule?
  • What is the sac of connective tissue surrounding the heart called?
  • A solution with a concentration of 0.25 mg/mL is used to deliver a 2 mg dose. How many milliliters are required?
  • Breeding outbred stocks results in which characteristic?
  • What induces the production of antibodies?
  • Which of the following statements best describes Isoflurane in a perioperative setting?
  • Environmental enrichment logs are an example of which type of record?
  • What does the left atrium do?
  • Which gland's regulation affects blood calcium levels?
  • Under anesthesia, what is required to ensure patient safety?
  • What is the primary purpose of the postapproval monitoring program?
  • Which animals are NOT required by law to be accompanied by a health certificate when being shipped?
  • Which side effect is associated with chemicals used to induce immunodeficiency?
  • Intraperitoneal injections are administered into which abdominal area?
  • Which two organizations require retention of IACUC minutes and semi-annual inspection reports?
  • Which action should be avoided to maintain sterility during gowning?
  • Which of the following is NOT a core value regarding the ethical treatment of animals?
  • Which instrument measures humidity?
  • Which forceps are designed to clamp onto bleeding tissue containing a severed vessel?
  • What does a B cell defect result in?
  • A negative result associated with multifilament suture material is:
  • What is a biologic filter?
  • Which committee reviews all protocols involving animals and oversees the animal care program?
  • During which estrous stage do ovaries contain developing ova?
  • What can be implanted for long-term, continuous infusion of a solution to unrestrained laboratory animals?
  • Which amount of calcium chloride would be needed to make a 1 molar solution in 1 L?
  • Which sampling method is commonly used to detect intestinal parasites by microscopic examination of feces?
  • During preoperative skin preparation, how should scrubbing be performed?
  • What is the smallest sized particle that HEPA filters are able to remove?
  • Which statement about trapped animals is FALSE?
  • Which technique uses a fine glass pipette to deliver DNA into the nucleus of a fertilized egg?
  • How do rodents respire?
  • The endocrine system's function includes which of the following statements?
  • If signs of poisoning occur after topical parasite treatment, which action is appropriate?
  • Which approach is recommended when organizing a to-do list?
  • A veterinarian prescribes for a 35 g mouse a 2 mg/kg dose. The medicine strength is 0.1 mg/mL. How many mL will you administer?
  • How many generations of brother-to-sister matings are needed to establish an inbred strain?
  • Diseases that are consistently present within a population are described as:
  • Which practice is TRUE about maintaining sterility during a procedure?
  • Which term describes the natural allele form as it occurs in nature, without mutation?
  • Which statement about amphibians and toxins is true?
  • What is an animal called that spreads an infectious disease while showing no signs of disease?
  • What is the role of hemoglobin molecules?
  • A quality assurance unit is responsible for ensuring compliance with which regulations?
  • What is the prescription for drug dose, frequency, and duration called?
  • Parturition refers to which biological event?
  • Which two species are most often used for acute toxicity testing?
  • Nutrients are used by the body for which purposes?
  • Which term describes the daily cost assigned to sustain one animal or cage for a day?
  • What is TRUE about clinical disease?
  • What does the Institutional Animal Care and Use Committee (IACUC) have the authority to do?
  • What is the process called by which you can determine the specific genetic makeup of an animal?
  • What is a biofilm?
  • What is a key difference in technique between intravenous injection and intravenous blood collection?
  • Urine flows from the kidneys to the urinary bladder through which structure?
  • Which of the following is responsible for production of gametes and secretion of sex hormones?
  • A 20 kg dog requires 5 mg/kg of a drug. If the drug is supplied as 50 mg/mL, how many milliliters are needed?
  • Neutrophils, eosinophils, and basophils are classified as which of the following?
  • Which is the smallest needle?
  • In a biologic filtration system, the substrate's main purpose is to provide a surface for nitrifying bacteria to colonize.
  • A common potential outcome of acute diarrhea in animals is what?
  • Which of the following is NOT a way suture material can be classified?
  • Under high ionic concentration in the water, osmosis tends to move water in which direction relative to the organism?
  • What is the role of a chemical filter in an aquatic system?
  • Why is food not withheld from rodents and rabbits before surgery?
  • Which action best supports maintaining sterility during surgery?
  • Which temperature range is commonly used in many facilities?
  • What is the predominant material found in roughage?
  • Which technique collects the embryonic stem cells that line the inside of a developing blastocyst to produce embryonic stem cell lines?
  • Catheters are placed in which of the following locations?
  • What occurs in pseudopregnancy?
  • What lighting level do most fish require?
  • What is the area of a cage that is 10 inches wide and 28 inches long?
  • The central nervous system consists of which components?
  • Which of the following is an example of an involuntary muscle contraction?
  • What is the approximate weaning age of most laboratory rodents?
  • Which system contains the brain and spinal cord?
  • In the designation B6C3F1/Crl, which segment indicates the maternal lineage?
  • How are spontaneous cancer models used?
  • Which guideline helps determine the appropriate syringe size relative to the fluid volume to be collected or injected?
  • What term describes the esophagus' movement that pushes food toward the stomach?
  • Diseases animals are born with are termed:
  • Mycoplasma pulmonis disease risk increases under high levels of which atmospheric component?
  • A tissue is defined as which of the following?
  • Which species has a palatal ostium?
  • Which of the following is TRUE about the ethical decision-making process?
  • What is the maximum amount of blood that should be taken from a 3 kg rabbit every 2 weeks?
  • How far above the floor should electrical outlets be located?
  • Into what part of the body is a subcutaneous injection given?
  • What are the first two cervical vertebrae called?
  • Which substance is an example of a chemical filter?
  • Which statement describes the distinction between a sanitizer and a disinfectant as provided?
  • What procedure will make the vessel more visible for an IV injection?
  • In GLP, which of the following is a correct description of a requirement?
  • Which advantage does a timed mating system provide?
  • Which of the following is the least potent sterilizer?
  • What does IACUC stand for?
  • If you have 22 sheep and each consumes 1.5 kg of feed per day, how many 50-lb bags of feed are needed per week?
  • Utility costs would be an example of which type of cost?
  • How often do the Animal Welfare Regulations require the IACUC to monitor the animal care and use program through reviews of the program and routine inspections of the animal facilities?
  • What is an epizootic disease?
  • A potential drawback of multifilament sutures is that they can harbor bacteria along the filament.
  • What is the primary function of pleural fluid in respiration?
  • Which type of tumor does not spread?
  • Which of the following statements is FALSE in regards to ethics and animal research?
  • Which chamber of the heart pumps blood to the body?
  • Which Act governs the care and use of animals in research at the federal level?
  • What does positive air pressure within a room do?
  • According to the Good Laboratory Practices Regulations, what are the observations, measurements, and specimens related to a study called?
  • How much dissolved oxygen is normally contained in water (by volume)?
  • In small animal anesthesia, which bedside sign is used to assess peripheral perfusion?
  • Which combination of steps is commonly used to improve venous visibility for IV injections?
  • Under the Animal Welfare Act, enrichment is required for which group?
  • What is the name of the technique for injecting DNA directly into the fertilized egg with a tiny glass pipette?
  • What is a zoonotic disease?
  • If both alleles of a gene pair are the same, an individual is considered to have which genetic makeup?
  • Which item can be used in place of sutures to close a wound?
  • Which term describes the destruction of red blood cells?
  • The Limulus amebocyte lysate test is used to assess which kind of contaminants?
  • Transmission from parent to offspring is called:
  • Which description best describes gas bubble disease in fish?
  • How many categories are there in the USDA pain descriptions?
  • What is the term that refers to the cost charged to maintain one unit, either one animal or one cage of animals, for one day?
  • The immediate management of suspected poisoning following topical parasite treatment should include which step?
  • What term describes slight withdrawal of a syringe plunger after placement of the needle?
  • The Animal Welfare Regulations specify that personnel should receive what type of training with animals with which they work?
  • Which of the following can be a sign of otitis?
  • Convert 18 inches to centimeters?
  • In the nitrogen cycle, which compound is produced when ammonia is oxidized by nitrifying bacteria?
  • What is the process in which bacteria breaks down roughage called?
  • Catgut sutures are an example of which suture category?
  • Which term describes having two different alleles at a gene locus?
  • Which antiseptic solution is commonly used for preoperative skin disinfection in the material?
  • What is the key routine for keeping an emergency cart ready for use?
  • Into what quadrant is an intraperitoneal injection given?
  • Which term describes a disease that can be transmitted from animals to humans or vice versa?
  • What is a grant application?
  • What is the process called when ammonia is converted to nitrite by bacteria in aquatic systems?
  • Indirect costs are best described as expenses that ...
  • What is a ganglion?
  • Which body system contains tissue that can be found in striated, smooth, or cardiac forms?
  • If an animal is homozygous null for a pair of genes, which term describes the animal's genetic modification?
  • What shape is the rodent kidney?
  • The basic functional unit of the kidney that filters blood is the
  • Which timeframe is typical for establishing a healthy biological filter with the appropriate bacteria?
  • Who should be responsible for proper documentation of animal care and use programs?
  • What step must occur before a research study using animals begins?
  • Which substance can be added to raise the pH?
  • Which choice is an injectable local anesthetic?
  • Which item is described as a form of structural enrichment?
  • Which of the following is TRUE about local anesthesia?
  • A 40 lb dog requires a dose of 20 mg/kg using a 15% solution. How many milliliters should be drawn up?
  • When is it safe to remove the endotracheal tube?
  • Which statement about absorbable sutures, such as catgut, is true?
  • Which breeding system involves parents that are from different inbred strains?
  • Which organization uses the acronym AAALAC?
  • What guideline assists in the selection of the size syringe to be used?
  • Which of the following terms is defined as "difficult birth"?
  • Which of the following is defined as a state of unconsciousness in which there is loss of sensation throughout the body?
  • Which substance that will destroy or inhibit growth of microorganisms and can be used on living tissue?
  • What is the period of time called that a chemical is left on a surface before it is rinsed?
  • What is the average amount of feed eaten by a nonhuman primate over a 3-day period if the amount of feed consumed over those days was 492 g, 499 g, and 500 g?
  • In a 3 kg rabbit, the maximum safe blood draw every two weeks is approximately 30 mL.
  • What membrane attaches tendons to the long bone?
  • Which system allows repeated vascular access without restraining or anesthetizing the animal each time?
  • What is the typical role of the Institutional Animal Care and Use Committee (IACUC) in research involving animals?
  • In the Guide, what word is used to describe aspects of an animal care program that are strong recommendations?
  • Suture material can be classified by which of the following aspects?
  • Which vendor produced the C57BL/6J mouse?
  • Which of the following is a step used in ethical decision making?
  • Whose responsibility is the humane treatment of animals in an animal facility?
  • Which of the following is a sign of severe pain?
  • What type of mating occurs when only 1 female and 1 male are put together in a cage?
  • Where does food begin its chemical digestion?
  • Under which policy is the five-member IACUC requirement specified?
  • What document published by the Institute of Laboratory Animal Research provides guidance on creating and implementing an institutional occupational health and safety program?
  • For proper sterilization of instruments in an autoclave, which practice is recommended?
  • Which species is an omnivore?
  • What is the difference between a monoclonal antibody and a polyclonal antibody?
  • Which statement is true about the mammalian heart?
  • What is the name of the policy that an institution has in place to protect individuals who report animal welfare concerns?
  • Which statement describes curved needle classification?
  • To reconstitute a 1 g vial of a freeze-dried drug to yield a 4% solution, what volume of diluent do you need to add?
  • What does a tether system do?
  • Which of the following destroys or inactivates infectious fungi and bacteria but not necessarily spores?
  • Using the conversion factor of 2.2 pounds per kilogram, how many pounds are in 5 kilograms?
  • A teratogen is best defined as which?
  • Segment II studies are primarily used to evaluate which toxicity aspect?
  • What does pH measure in the aquatic system?
  • What is the most effective method to prevent fighting in group-housed animals?
  • Which lymph nodes are found in the neck?
  • When an animal is suspected of having a disease, in which housing area should it be placed?
  • How many Institutional Animal Care and Use Committee (IACUC) members are required by the Animal Welfare Regulations (enforced by the United States Agricultural Department)?
  • What is pH?
  • What is the valve between the left atrium and the left ventricle of the heart?
  • The process of forming a blood clot is called what?
  • Which instrument family includes devices used to control bleeding during surgery?
  • A drawback of using absorbable catgut sutures is:
  • When should husbandry for quarantined animals be scheduled?
  • How often must the controlled substance inventory list be updated?
  • Which animals are covered under the Public Health Service (PHS) Policy?
  • If an animal used in experiments was given pain-relieving drugs, which USDA pain category would describe this?
  • What is homeostasis?
  • What is the first step in reporting noncompliance?
  • Gastric gavage is best described as delivering a liquid to which location via a tube?
  • Before a new drug is tested on human subjects, the drug is tested on animals in a phase that is called __________ testing.
  • How many times a day would you administer a drug prescribed to be given QID?
  • Which of the following could contribute to a flooded cage?
  • Which of the following has a negative effect on breeding?
  • What does the term medial mean?
  • Which statement is FALSE for maintaining sterility?
  • Gastric gavage needle length is determined by measuring from which anatomical landmark?
  • Which of the following is NOT one of the three periods of surgery?
  • Which document is commonly used as the standard for humane care and use of laboratory animals in federally funded research?
  • Which functions are regulated by hormones produced in the posterior pituitary gland?
  • What surgical instrument is a rigid cutting device used for incising or puncturing tissue?
  • What do pheromones do?
  • What is the relaxation phase of the heart called?
  • Why must ultraviolet germicidal light operate within a specific wavelength range?
  • In the digestion of roughage, which compartment in the goat's stomach is most associated with fermentation by microorganisms?
  • Which parameter is a measure of both calcium and magnesium ions in water?
  • Which animal lacks both B cells and T cells?
  • Which molecule within red blood cells carries respiratory gases?
  • What step does the assistant perform to aid with gowning?
  • Which is the main shaft of the long bone?
  • When a cage becomes flooded, which of the following could be causes?
  • Which adverse outcome is listed as a side effect of chemicals used to induce immunodeficiency?
  • Which process describes the conversion of ammonia to nitrite and nitrate in aquatic systems?
  • What is the Whitten Effect?
  • Which statement is true about pheromones in rodents?
  • Which lists routes of injection from fastest absorption to slowest?
  • Which parameter is a measure of the amount of dissolved salts and minerals, present as ions, within an aqueous solution?
  • How is needle length determined when performing a gastric gavage?
  • A bag of feed weighs 15 kg and costs $50. What is the cost per kilogram?
  • What is active immunity?
  • Which provision is NOT required by Public Health Service Policy?
  • If the air pressure inside the animal room is lower than the air pressure in the surrounding rooms, the room is said to be under:
  • Which of the following is NOT a component of animal cells?
  • What helps to prevent cell lysis during blood collection?
  • Which biosafety level is considered the most basic containment with no risk to personnel?
  • What is a chimera?
  • Which mandate requires five Institutional Animal Care and Use Committee (IACUC) members?
  • How are curved needles classified?
  • In containment, what is the term for air pressure that is intentionally lower inside a room than in adjacent spaces?
  • Why should an unconscious, postoperative animal be turned frequently from side to side?
  • Where does each bronchus branch out to?
  • What is a major advantage of using gas anesthesia compared with injectable anesthesia?
  • How much of the tail may be snipped with performing a tail transection?
  • Which hormone lowers blood glucose?
  • Which of the following is a disinfectant?
  • What is the correct order of testing when developing a new drug?
  • In GLP terminology, which term describes the data that are collected and used to assess the study's outcomes?
  • Which statement best describes the requirement before starting animal research?
  • What is the name of the subdivision of the peripheral nervous system that regulates involuntary functions of internal organs?
  • Which biosafety level is the most basic level of containment and there is no risk to personnel?
  • Which function is associated with hormones produced in the adrenal gland?
  • What is the purpose of the expansion colony?
  • The presence of a vaginal plug in a female mouse or rat most likely indicates what?
  • A cut-down procedure is used to visualize a blood vessel for catheterization.
  • The use of controlled substances in an animal facility is controlled by which organization?
  • Which costs are consistent throughout the budget period?
  • What condition is commonly observed in rabbits after transport?
  • What is the term that refers to genes that are located adjacent to one another on the same chromosome that tend to be inherited together?
  • Nitrite in the nitrogen cycle is best described as:
  • Which system runs a constant stream of water into and out of each tank without water recirculation?
  • How does irradiation attack the immune system?
  • What is formed when stomach contents mix with acidic gastric secretions?
  • Which of the following is an example of a primary barrier?
  • What is the stage of estrus in which ova develop in the ovary?
  • Which statement best reflects the goal of welfare in animal research?
  • Which organ produces bile, breaks down chemicals, and stores glycogen?
  • Which term best describes the area prepared to maintain sterility during an operation?
  • What is a tissue?
  • What action helps ensure the emergency cart is not depleted during an emergency?
  • The term 'medial' describes a location toward what?
  • What causes the mitral valve to open?
  • What occurs when an animal goes into shock?
  • What is another name for the gastrointestinal (GI) tract?
  • Which statement best describes the condition commonly known as bloat?
  • Which membrane covers the outer surface of a bone and serves as a site for tendon attachment?
  • The endotracheal tube's primary purpose is to provide a secure airway for delivering which of the following?
  • Which term describes a variant form of a gene?
  • Which of the following is not a standard term used to name the digestive tract?
  • What is lymph derived from?
  • What are the short, multi-branched extensions on neurons called?
  • What term defines a substance that increases the immune response to an antigen?
  • Polyclonal antibodies are typically produced by
  • What information is NOT provided in a grant application?
  • In a long bone, which region is the main shaft?
  • Which statement best characterizes decentralized management in animal facilities?
  • During the establishment of a biologic filter in an aquatic system, which substances may rise and remain elevated?
  • What does the word coagulation refer to?
  • Which two federal agencies require compliance with Good Laboratory Practice Regulations?
  • In anesthesia, which equipment should be readily available to secure an airway?
  • Which option best helps prevent damage to cells during blood collection?
  • If animals are to be euthanized at the end of a study, the method of euthanasia must be in accordance with the guidelines set up by the ____________.
  • Which policy governs use of vertebrate animals in federally funded research?
  • What is a recommended practice to maintain emergency cart readiness?
  • Inflammation can cause edema, which is best defined as which of the following?
  • What is the spectrum of activity?
  • The parathyroid gland regulates which of the following?
  • What is the process called when fertilized eggs or embryos are taken from a female and implanted into a female who has been tested and found free of pathogens?
  • The observable traits of an organism are studied by which process?
  • Which should pups NOT be in direct contact with?
  • Exophthalmia is another term used to describe which condition?
  • Which feature is required to be provided for dogs by the AWA?
  • Amphibians and fish have permeable skin which can allow which of the following to happen?
  • Which statement about anesthetic emergencies is FALSE?
  • What must be done to instruments that have been sterilized with aldehydes before using them?
  • What usually helps determine animal facility design?
  • What is the primary purpose of a cut-down procedure in catheterization?
  • Which term refers to the complete removal of all visible debris from a surface?
  • What process occurs during electroporation?
  • Which period of surgical care occurs during the procedure itself?
  • If an animal shows signs of poisoning shortly after being treated with a topical powder for external parasites, what should be done?
  • What is the name of the program that helps to ensure that IACUC-approved procedures are being followed?
  • Active immunity refers to which immune response?
  • Which of the following is NOT toxic to amphibians?
  • What is the name of a device used to cannulate a blood vessel?
  • Which term describes a tank system that does not exchange water with the environment?
  • What does it mean if an animal is considered to be a wild type animal?
  • What are activities or entities that have expenses but do not directly produce revenue?
  • Which of the following should be used to help protect immunocompromised animals?
  • What is the agreement from a research institution outlining how it complies with the Public Health Service Policy?
  • Which substance is described as a disinfectant in the material?
  • Which organization is typically responsible for reviewing and approving animal use protocols before research begins?
  • Which method of monitoring the effectiveness of sanitation or sterilization uses spores in a vial?
  • What does hemolysis refer to in laboratory terminology?
  • A cluster of nerve cells is called a
  • Adding sodium bicarbonate to raise pH will primarily affect which parameter?
  • What may induce cancer?
  • Which tool might be needed to release a trapped animal?
  • What percentage of total blood volume is comprised of erythrocytes, leukocytes, and thrombocytes?
  • Which system provides shape and protection?
  • In rodent mating, the behavioral posture described during estrus is called what?
  • Which statement best describes the role of ethics in research?
  • What is a gene referred to that is absent or turned off due to a mutation that blocks the gene's expression?
  • Why is it recommended to have an emergency plan in place for anesthesia?
  • What is an adjuvant?
  • What is the term for a fetus obstructing the birth canal?
  • How is an animal's antibody titer raised further after the initial injection of antigen?
  • How many years does a typical grant provide money for funding of a research project?
  • What is the typical duration of a subchronic toxicity test?
  • What term describes the brief stressful period experienced by aquatic organisms while the biologic filter is being established?
  • Which statement best explains why different per diem rates appear across institutions?
  • Postpartum estrus occurs after birth in which species?
  • What is a diagnosis?
  • Ataxia is defined by which of the following?
  • What is the term used to describe a situation where new genes are inserted by a targeted mutation?
  • Which can help determine what services are cost effective?
  • Which statements describe the hormones produced in the adrenal gland?
  • What is a metabolism cage used for?
  • Which term describes the act of slight withdrawal of the syringe plunger after needle placement?
  • To reduce unauthorized access to an emergency cart when it is not in use, which practice is recommended?
  • A tumor that remains localized and does not invade surrounding tissues is described as:
  • What is the concept that states that people behave based on commonly held core values?
  • Which statement is TRUE?
  • Which of the following factors of the decontamination or sterilization agent will NOT determine success?
  • Which term describes the passage of a newly introduced gene through the reproductive cells to future generations?
  • Under the Animal Welfare Act (AWA) and Animal Welfare Regulations (AWR), which animal is covered?
  • Which of the following is a liquid chemical ('cold') sterilizing agent?
  • What will happen to an aquatic species if the water in the system has too many ions?
  • Which of the following is FALSE about nonintensive breeding?
  • What is it called when a new gene is incorporated into the germ cells (egg and sperm)?
  • Hormones produced by the posterior pituitary gland regulate water resorption and smooth muscle contraction. Which gland produces these hormones?
  • Which of the following is the definition for per diem rate?
  • Which statement describes the effect of irradiation on immune cells?
  • Which of the following is a Good Laboratory Practice (GLP) requirement?
  • Which option best describes the genome?
  • What is the term for induction of an increased number of eggs that mature and are released from the ovaries simultaneously?
  • The process of identifying a disease is known as what?
  • Which test is commonly used to detect internal parasites in animals?
  • What is the mechanism for gas exchange in the body?
  • Otitis is a condition involving inflammation of which part of the body?
  • Who can restrict or withdraw approval of the Animal Welfare Assurance if an institution fails at its responsibilities?
  • Which of the following reviews the animal use protocols to ensure compliance with all relevant federal, state, and local laws, regulations, and institutional policies on animal welfare?
  • Which type of radiation is considered the least potent sterilizer among the listed options?
  • During surgical hand scrubbing, why is a new piece of gauze used for each scrubbing cycle?
  • What unit is used to express light level in aquarium lighting?
  • Why would rederivation be used?
  • Which statement about water treatment in aquatic systems is true?
  • Which muscle type has voluntary movement?
  • What type of costs are associated with a specific research project?
  • Which of the following is a frequent cause of a flooded cage?
  • To establish an inbred strain, matings are typically done between which relatives?
  • What relative humidity range does the Guide recommend for most mammalian species?
  • Which type of disease is caused by a pathogen and can be transmitted between individuals?
  • Alopecia is best defined as which condition?
  • Why can per diem rates differ between institutions?
  • Conductivity indicates what in water?
  • What term describes a genotype with identical alleles at a locus?
  • Which statement about PHS Policy coverage is accurate according to the material?
  • Which animal may have blood drawn from the orbital plexus?
  • What should be done if resistance is felt while inserting a gastric gavage tube?
  • Which structure filters blood, forming urine with water and wastes removed from the blood?
  • What is the process called in which a reward is used to get an animal to repeat a behavior?
  • Multi-dose toxicity testing is primarily conducted to study what?
  • In which species are red blood cells nucleated in adulthood?
  • What is research?
  • What process is a standard way to dechlorinate water?
  • Which 3Rs principle would be applied by replacing animals with non-animal alternatives?
  • What is the term for the posture a female in estrus stands when near a male?
  • What is the function of retractors in surgery?
  • Which term describes the study of moral principles guiding behavior in research contexts?
  • What is the term for a hollow tube through which blood or other body fluids may be withdrawn or substances administered?
  • Which is NOT a bone in the forelimb?
  • What is the first step in scientific research?
  • Which term describes something present at birth?
  • Which statement is true about thermometers?
  • Which of the following is NOT a type of blood vessel?
  • Where are the popliteal lymph nodes located?
  • Which hormone lowers the blood glucose level?
  • What does IACUC stand for?
  • What is the correct sequence when treating an abscess?
  • What causes a hematoma?
  • Why do arteries have thicker walls than veins?
  • Which statement about mastitis is TRUE?
  • A drug concentration is 25 mg/mL. If you need a 200 mg dose, how many milliliters should you draw up?
  • The mitral valve connects which two chambers?
  • Gas bubbles formed during gas bubble disease primarily occur in which parts of the body?
  • Removal of which gland leads to a deficiency in T cells?
  • A teratogen is defined as what?
  • For which nutrient study would the rat NOT be an appropriate model?
  • What is a mammalian equivalent of the cloaca?
  • Which condition is related to an abnormal amount of air, food, or liquid in the stomach?
  • Excessive fear is an example of which phenotype?
  • Which is an example of a record that animal facilities are required to keep up-to-date?
  • What movement occurs when a bone is moved away from the midline of the body?
  • Which gland can be surgically removed to cause a deficiency in T cells?
  • What is the route of administration for a drug prescribed to be given ID?
  • What is the term for a local swelling that may occur as a result of inflammation?
  • What are all the genes in an organism collectively called?
  • What is the formula for the area of a room or cage?
  • What is the definition of compliance?
  • What is the predominant carbohydrate found in roughage?
  • Which filter medium is commonly used to remove dissolved chemical contaminants from aquarium water?
  • Which circulating cells contain hemoglobin molecules?
  • Which description best fits a cross where the parents are from different inbred strains?
  • Which process ensures laboratory animal programs meet established standards for care and use?
  • Which practice is NOT recommended for maintaining an emergency cart?
  • Which chamber of the heart receives blood from the lungs?
  • What is the primary purpose of a sharps container?
  • Which layer forms the outer protective barrier of the skin?
  • Which of the following is an infectious disease agent?
  • The Animal Welfare Regulations require that protocols for United States Department of Agriculture (USDA)-covered species may only be approved for how long?
  • Which part of the strain name is the maternal strain for the B6C3F1/Crl mouse?
  • What ingredient does Freund's Complete Adjuvant contain that is responsible for undesirable side effects?
  • What is a substance that produces a fever called?
  • What process can be used to preserve a genetic line by freezing embryos?
  • After surgery, needles and disposable sharp objects should be disposed of in:
  • If an animal used in teaching, research, experiments, or tests causes no pain or distress and does not require pain-relieving drugs, what USDA pain category would this describe?
  • What is aseptic technique?
  • A metabolism cage is used to measure which of the following parameters?
  • Why is proper recordkeeping important in animal research?
  • What is the gauge of the needle?
  • Macaques with mouth ulcers may indicate infection with which disease?
  • What type of filter primarily removes large, solid waste from water?
  • Alopecia refers to which symptom?
  • If an animal experiences shock, which outcome is most accurate?
  • Which condition is characterized by a lack of voluntary muscle coordination?
  • Which 3Rs principle is being applied when a researcher minimizes the number of potentially painful procedures that animals must undergo?
  • What method can sterilize instruments in 10 seconds?
  • What is the approximate blood volume of an animal?
  • Which floor plan design uses exit doors that lead from each room to a dirty corridor that wraps around the floor plan?
  • What type of management program usually has the animal care program in a separate department that serves all the institution's animal care needs and requests?
  • What may induce cancer?
  • IACUCs are mandated by which of the following?
  • The spectrum of activity describes which of the following?
  • Pseudopregnancy involves signs of pregnancy without which event?
  • A 2.0 kg animal requires 0.75 mg/kg of a medication. If the solution is 10 mg/mL, how many milliliters should be administered?
  • Which term describes the fluid component inside the cell that contains organelles and cytosol?
  • A medication has a concentration of 50 mg/mL. How many milliliters should a 500 g guinea pig receive if the dosage is 10 mg/kg?
  • A solution with a concentration of 2 mg/mL is used to deliver a 12 mg dose. How many milliliters are required?
  • What is it referring to if scissors are classified as blunt-blunt?
  • Which group of animals is known to have concentrated urine that is almost solid and white?
  • After how many litters is it recommended that female mice be retired as breeders?
  • Which type of transmission occurs among animals in the current generation in a facility?
  • What is the germicidal spectrum of ultraviolet light for killing microorganisms?
  • Which organ is involved in detoxification and metabolism and stores glycogen?
  • Which mouse strain is described as lacking both B cells and T cells?
  • What is an example of the 3Rs principle of reduction?
  • Which of the following is NOT true about endotracheal tubes?
  • Which factor most influences how an animal facility is designed?
  • What is the difference between genotype and phenotype?
  • The USDA requires the tags of which animal be retained for a year?
  • A subchronic toxicity study typically lasts about how long?
  • How does the Guide define biosecurity?
  • What term describes a passageway with two locking doors which cannot be opened simultaneously?
  • What is a complicating factor in urinary catheterization?
  • What housing features are typically needed for newborn piglets?
  • Which lymph node group is located in the groin region?
  • What must a facility create and follow as required by the Good Laboratory Practice (GLP) Regulations?
  • Which term best describes maintaining a stable internal environment?
  • Which item is NOT typically needed when interacting with people in an animal facility?
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